Title of the tutorial (it will appear on the tutorial page and the topic page)
Example(s)
title:Clustering in Machine Learning
title:Breve introducción a Galaxy - en español
title:Pangeo ecosystem 101 for everyone - Introduction to Xarray Galaxy Tools
contributions
A dictionary/map
List of tutorial contributors. Here we break them down into several broad categories to help contributors identify how they contributed to a specific tutorial.
List of tutorial contributors. Please use contributions instead as it provides more detailed accounting of tutorial history.
Example(s)
contributors:-hexylena-shiltemann
Free Text
An edam ontology id that describes the topic or tutorial.
Example(s)
edam_ontology:topic_3173
Required Pattern: Must match the following regular expression
/^topic_[0-9]+$/
Boolean
false to hide your tutorial from the topic page (optional). This is useful if you need a tutorial for a workshop, but have not finished making it up to GTN standards.
List of Items
list of resources that the reader of the material could follow at the end of the tutorial
[Internal Only] List of required tutorials inside that topic
Decimal Number
Currently unused.
Free Text
Link to a gitter channel that is more relevant for the tutorial than the default. E.g. a single cell tutorial could use Galaxy-Training-Network/galaxy-single-cell to link to their specific chat room.
Example(s)
gitter:Galaxy-Training-Network/galaxy-single-cell
gitter:galaxy-genome-annotation/Lobby
List of Items
List of take-home messages. This information will appear at the end
of the tutorial. These should really be a key point, something that
should stick in their mind; what you want them to take home from
the tutorial.
Example(s)
key_points:-Pangeo ecosystem enables big data analysis in geosciences-The MiModD suite of tools bundles most of the functionality required to perform mapping-by-sequencing analyses with Galaxy-It can drastically simplify management of large numbers of VMs
Free Text
The document language.
Possible Values:
es
en
Example(s)
lang:"es"
lang:"en"
Free Text
Here give a feeling of what level the material is at.
Possible Values:
Introductory
Intermediate
Advanced
Example(s)
level:"Introductory"
level:"Intermediate"
level:"Advanced"
Free Text
An SPDX identifier for the alternative license that is used for that particular material. This is only relevant for materials which have been imported from an external source and were originally licensed under another license. For new materials we strongly encourage contributors to not use this key as materials are CC-BY, by default.
However, it means we can run a lot of our Python training
directly in the GTN! And in the future, hopefully, we will
be able to embed individual cells of the notebook directly
in the Python training, so the user doesn’t even need to
switch pages.
Enabling this field will enable pyolite links for this notebook.
Free Text
If you have an alternative preamble for your notebook that students should know before following (e.g. they must load X datasets in their history), it can be listed here.
This text will be shown in the GTN tutorial, but it will not be included in the notebook, giving you a bit better control over mixing setup content which is relevant for Galaxy, with notebook content that can be easy to run for students.
A learning objective is a single sentence describing what a learner will be able to do once they have done the tutorial. Generally it is best to follow a 2C or 3C learning objective such as:
Compute (Skill)
multiple whole genome assemblies (Objective)
in such a way to develop big data processing skills (Result)
Example(s)
objectives:-Understand the basic concepts behind phylogenetic trees, as applied to *Mycobacterium tuberculosis*-Explore Biodiversity data with taxonomic, temporal and geographical informations-Generate a DotPlot emulating the original paper using a different analysis tool
Integer Number
This field allows ordering tutorials within the tutorial list. Tutorials with lower numbered priority come before tutorials with higher numbers.
Example(s)
List of Items
list of questions that will be addressed in the tutorial
Example(s)
questions:-How does Genome assembly work?-How do I change Galaxy configs?-How to detect and quantify differentially abundant proteins in a HEK-Ecoli Benchmark DIA datatset?-What kinds of data do programs store?
List of Items
If a tutorial is renamed to a new location, use this field to list prior locations from which this tutorial was accessible.
List of resources that the reader of the material should be familiar with before starting this training. The structure is identical to follow_up_training.
[Internal Only] List of required tutorials inside that topic
Free Text
if the topic has multiple subtopics defined, you can assign your tutorial to one of those subtopics here. Without this, the tutorial will appear in the “Other tutorials” section on the topic page.
Example(s)
subtopic:single-cell
List of Items
A free form list of tags that are relevant for your tutorial.
Example(s)
tags:-covid-19-git-gat
List of Items
If alternative translations of a material are available, then use this key to indicate which languages have been manually translated.
Example(s)
translations:-en
A dictionary/map
For materials which are automatically converted into videos via the available mechanisms, this field declares which voice should be used. If this field is not declared, a random voice will be chosen from a list of the best available voices from AWS Polly.
Example(s)
voice:id:Lupelang:es-USneural:true
id
Free Text
lang
Free Text
neural
Boolean
Free Text
link on Zenodo to the input data for the tutorial
Example(s)
zenodo_link:https://zenodo.org/record/3706539
A dictionary/map
Example(s)
hexylena:name:Helenatwitter:hexylenabio:I wrote this documentation! I do super cool things.
A dictionary/map
This ideally is your GitHub handle. If you do not have, or do not wish to provide a GitHub username, you may make up another identifier here, but then you must set github: false as described below.
name
Free Text
Your preferred name. If you prefer an alias or another name, this is welcome, it does not need to be your legal name.
Example(s)
name:张三
name:Alice
name:Jane Doe
name:Madame Tout-le-Monde
name:Γιάννης Παπαδόπουλος
joined
Free Text
The year and month in which you joined
Example(s)
joined:2020-01
Required Pattern: Must match the following regular expression
/[0-9]{4,}-[0-9]{2}/
Free Text
Free Text
A short biography of yourself, if you wish to add additional details or context.
Example(s)
bio:Research at the [South African National Bioinformatics Institute](https://www.sanbi.ac.za/)
Free Text
The 2 letter code identifying the ELIXIR node to which you are a member or are associated with. If you are from norway, you will need to quote your value, "no", unlike everyone else, due to the Norway Problem with YAML
Possible Values:
au
be
ch
cz
de
dk
ee
es
fi
fr
gr
hu
ie
il
it
lu
nl
no
pt
se
si
uk
Example(s)
elixir_node:"au"
elixir_node:"be"
elixir_node:"ch"
elixir_node:"cz"
elixir_node:"de"
elixir_node:"dk"
elixir_node:"ee"
elixir_node:"es"
elixir_node:"fi"
elixir_node:"fr"
elixir_node:"gr"
elixir_node:"hu"
elixir_node:"ie"
elixir_node:"il"
elixir_node:"it"
elixir_node:"lu"
elixir_node:"nl"
elixir_node:"no"
elixir_node:"pt"
elixir_node:"se"
elixir_node:"si"
elixir_node:"uk"
Free Text
Your email address, if you wish to provide it.
Example(s)
email:jane.doe@gmail.com
Required Pattern: Must match the following regular expression
/@/
Boolean
Set this to true if this entity is a funding agency.
Free Text
The short identifier for your grant.
Example(s)
funding_id:2020-1-NL01-KA203-064717
Free Text
A short statement about the funder, markdown is supported.
Example(s)
funding_statement:This project ([`2020-1-NL01-KA203-064717`](https://ec.europa.eu/programmes/erasmus-plus/projects/eplus-project-details/#project/2020-1-NL01-KA203-064717)) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.
Boolean
If your identifier in this file is not a GitHub account (or not your account), then this must be set to true, so we do not link to that account.
Free Text
Your gitter
Example(s)
gitter:hexylena
Required Pattern: Must match the following regular expression
/[0-9a-zA-Z]+/
Free Text
Set this to no if you would like to be excluded from the hall of fame.
Possible Values:
no
Example(s)
halloffame:"no"
Free Text
Required Pattern: Must match the following regular expression
/[0-9a-zA-Z]+/
Free Text
Preferred contact method
Free Text
Your matrix identifier
Example(s)
matrix:@hexylena:matrix.org
Required Pattern: Must match the following regular expression
/@[0-9a-zA-Z]+:.*/
Free Text
Your identifier at orcid.org
Example(s)
orcid:0000-0001-9760-8992
Required Pattern: Must match the following regular expression
/[0-9A-Z]{4}-[0-9A-Z]{4}-[0-9A-Z]{4}-[0-9A-Z]{4}/
Free Text
Your twitter handle, without the @
Example(s)
twitter:gxytraining
Required Pattern: Must match the following regular expression
/[0-9a-zA-Z]+/
A dictionary/map
layout
Free Text
The layout to use. Generally you should use tutorial_slides and no other value.
Possible Values:
base_slides
tutorial_slides
Example(s)
layout:"base_slides"
layout:"tutorial_slides"
title
Free Text
Title of the slides (it will appear on the slides and the topic listing)
Example(s)
title:Introduction to CRISPR screen analysis
title:High Performance Computing for Pairwise Genome Comparison
title:Circos
contributions
A dictionary/map
List of slide contributors. Here we break them down into several broad categories to help contributors identify how they contributed to a specific tutorial.
These entities wrote the bulk of the training material, they may have done the analysis, built the workflow, and wrote the text themselves.
List of Items
These entities edited the text, either for spelling and grammar, flow, GTN-fit, or other similar editing categories
List of Items
These entities provided funding support for the development of this resource
List of Items
These entities managed and provided infrastructure to the GTN or for training purposes
List of Items
These entities tested the tutorial to ensure it works correctly for students, or reported issues with the tutorial.
List of Items
These entities did translation and localisation work on this resource
List of Items
These entities contributed UX or Design improvements to this tutorial or the GTN as a whole
logo
Free Text
A logo identifier (e.g. GTN) should be used by default, but may be swapped out for special logos from the assets folder.
Possible Values:
GTN
assets/images/gat.png
assets/images/gga.png
assets/images/ncbi.png
/assets/images/elixir-uk.png
Example(s)
logo:"GTN"
logo:"assets/images/gat.png"
logo:"assets/images/gga.png"
logo:"assets/images/ncbi.png"
logo:"/assets/images/elixir-uk.png"
Free Text
A default CSS class applied to slides
List of Items
List of tutorial contributors. Please use contributions instead as it provides more detailed accounting of tutorial history.
Example(s)
contributors:-hexylena-shiltemann
Free Text
An edam ontology id that describes the tutorial.
Example(s)
edam_ontology:topic_3173
Required Pattern: Must match the following regular expression
/^topic_[0-9]+$/
Boolean
false to hide your tutorial from the topic page (optional). This is useful if you need a tutorial for a workshop, but have not finished making it up to GTN standards.
List of Items
list of resources that the reader of the material could follow at the end of the tutorial
[Internal Only] List of required tutorials inside that topic
Free Text
Possible Values:
external
Example(s)
hands_on:"external"
Free Text
List of Items
List of take-home messages. This information will appear at the end
of the slides. These should really be a key point, something that
should stick in their mind; what you want them to take home from
the slides.
Example(s)
key_points:-Pangeo is an inclusive community promoting open, reproducible and scalable science.-Balanced batches and replicates allow bioinformatic batch correction-Galaxy workflows can download images from the IDR, selecting specific channels, time points, z-stack positions and crop the image in different ways.
Free Text
The document language.
Possible Values:
es
en
Example(s)
lang:"es"
lang:"en"
Free Text
Here give a feeling of what level the material is at.
Possible Values:
Introductory
Intermediate
Advanced
Example(s)
level:"Introductory"
level:"Intermediate"
level:"Advanced"
List of Items
list of learning objectives for the tutorial
A learning objective is a single sentence describing what a learner will be able to do once they have done the tutorial. Generally it is best to follow a 2C or 3C learning objective such as:
Compute (Skill)
multiple whole genome assemblies (Objective)
in such a way to develop big data processing skills (Result)
Example(s)
objectives:-Understand the basic concepts behind phylogenetic trees, as applied to *Mycobacterium tuberculosis*-Explore Biodiversity data with taxonomic, temporal and geographical informations-Generate a DotPlot emulating the original paper using a different analysis tool
Integer Number
This field allows ordering resources within the topic list. Learning resources with lower numbered priority come before those with higher numbers.
Example(s)
List of Items
list of questions that will be addressed in the slides
Example(s)
questions:-What is ATAC-Seq?-What are Galaxy Interactive Environments (GIEs)?-How to visualize your genome after automated annotations have been performed?
List of Items
If a tutorial is renamed to a new location, use this field to list prior locations from which this tutorial was accessible.
List of resources that the reader of the material should be familiar with before starting this training. The structure is identical to follow_up_training.
[Internal Only] List of required tutorials inside that topic
Free Text
if the topic has multiple subtopics defined, you can assign your tutorial to one of those subtopics here. Without this, the tutorial will appear in the “Other tutorials” section on the topic page.
Example(s)
subtopic:single-cell
List of Items
A free form list of tags that are relevant for your tutorial.
Example(s)
tags:-covid-19-git-gat
Free Text
An estimation of the time needed to complete the hands-on. It should look like 10M or 1H30M
Required Pattern: Must match the following regular expression
If alternative translations of a material are available, then use this key to indicate which languages have been manually translated.
Example(s)
translations:-en
Boolean
This enables automatic slide-to-video conversion. See the documentation of that feature for more information about this feature.
If enabled, you must provide good speaker notes for every single slide.
A dictionary/map
For materials which are automatically converted into videos via the available mechanisms, this field declares which voice should be used. If this field is not declared, a random voice will be chosen from a list of the best available voices from AWS Polly.
Example(s)
voice:id:Lupelang:es-USneural:true
id
Free Text
lang
Free Text
neural
Boolean
Decimal Number
Decimal Number
Free Text
link on Zenodo to the input data for the tutorial
Example(s)
zenodo_link:https://zenodo.org/record/3706539
A dictionary/map
layout
Free Text
This must be set to faq
Possible Values:
faq
Example(s)
layout:"faq"
title
Free Text
Title of the FAQ
Example(s)
title:How does the GTN implement the 'Ten simple rules for collaborative lesson development'
title:How can I give feedback?
title:Ways to use Galaxy
box_type
Free Text
The type of box that should be used when rendering this FAQ. Use none if it should not be in a box, but just plain text.
Possible Values:
tip
hands_on
question
comment
warning
details
none
Example(s)
box_type:"tip"
box_type:"hands_on"
box_type:"question"
box_type:"comment"
box_type:"warning"
box_type:"details"
box_type:"none"
contributors
List of Items
Who contributed to this FAQ
Example(s)
contributors:-shiltemann-hexylena
Free Text
A text key used for sorting related FAQs together in the visual interface for users.
Example(s)
area:contributors
area:learners
area:features
Free Text
A short, one line description to provide additional context of the FAQ
Example(s)
description:Galaxy may have several reference genomes built-in, but you can also create your own.
description:Quickly learn what the identifiers are in any **BAM** dataset that is the result from mapping
description:Finding and Correcting Metadata
List of Items
If a tutorial is renamed to a new location, use this field to list prior locations from which this tutorial was accessible.
Required Pattern: Must match the following regular expression
/^topic_[0-9]+$/
Boolean
false to hide your topic from the production GTN. This is useful if you need a topic for a workshop, but have not finished making it up to GTN standards.
Free Text
Link to a gitter channel that is more relevant for this topic than the default. E.g. a single cell topic, you could use Galaxy-Training-Network/galaxy-single-cell to link to their specific chat room in all of the child tutorials by default.
Example(s)
gitter:Galaxy-Training-Network/galaxy-single-cell
gitter:galaxy-genome-annotation/Lobby
List of Items
Example(s)
references:--|authors: "Vaudel M, et al."title: "Shedding light on black boxes in protein identification."link: "https://www.ncbi.nlm.nih.gov/pubmed/24678044"summary: "An extensive tutorial for peptide and protein identification, available at http://compomics.com/bioinformatics-for-proteomics. The material is completely based on freely available and open-source tools."
Sequence Value (List of items)
A dictionary/map
authors
Free Text
title
Free Text
link
Free Text
Free Text
List of Items
List of resources that the reader of the material should be familiar with before starting this training. The structure is identical to follow_up_training.
For large topics, we can define subtopics and create multiple tutorial lists, which separates the tutorials to help users find content that interests them more quickly.
A dictionary/map
id
Free Text
Subtopic ID, this should match what is used in tutorials.
Example(s)
id:single-cell
Required Pattern: Must match the following regular expression
/^[A-Za-z0-9_-]+$/
title
Free Text
Subtopic title, which is displayed for users to see.
Example(s)
title:Maintaining a Production Galaxy
title:Single-cellRNA-seq
description
Free Text
A human readable textual description of a subtopic.
Example(s)
description:- "Start here if you are new to RNA-Seq analysis in Galaxy"-"Thesetutorialstakeyoufromrawsequencingreadstopathwayanalysis"-"Tutorialsaboutanalysisofsingle-cellRNA-seqdata"-"Tutorialsusingasinglepublishedsingle-cellRNA-seqdatasetforavarietyofanalyses"
Boolean
false to hide your topic from the production GTN. This is useful if you need a topic for a workshop, but have not finished making it up to GTN standards.
A dictionary/map
title
Free Text
Title of the Quiz
Example(s)
title:Diagnostic Assessment
title:SQL Basics Recap
contributors
List of Items
Who contributed to this FAQ
Example(s)
contributors:-shiltemann-hexylena
questions
List of Items
The questions that are part of the quiz
A dictionary/map
title
Free Text
The question title, displayed on the teacher and student screens simultaneously
Example(s)
title:How you could collect information from learners at the beginning of a course or lesson and how you can use it?
title:What keyword do you use to retrieve data in SQL
timeout
Integer Number
How long the students have to answer the question
type
Free Text
What type of question to display.
Possible Values:
choose-1
choose-many
free-text
poll
Example(s)
type:"choose-1"
type:"choose-many"
type:"free-text"
type:"poll"
List of Items
The possible answers to the question you’ve asked. Consider
including common wrong answers, or answers based on common
mistakes to help students identify where they went wrong.
Example(s)
answers:-OBTAIN-SELECT-RETRIEVE-RETURN
The correct answer, it MUST be part of the answers list. It can be a list of correct answers if it is a choose-many question
Example(s)
correct:SELECT
correct:AB
Free Text
Path to an image, if you’d like to include one along with the question. Should be the full path starting with /
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Batut et al., 2018 Community-Driven Data Analysis Training for Biology Cell Systems 10.1016/j.cels.2018.05.012
@misc{contributing-schemas,
author = "Helena Rasche",
title = "GTN Metadata (Galaxy Training Materials)",
year = "",
month = "",
day = ""
url = "\url{https://training.galaxyproject.org/training-material/topics/contributing/tutorials/schemas/tutorial.html}",
note = "[Online; accessed Sat Jul 19 2025]"
}
@article{Batut_2018,
doi = {10.1016/j.cels.2018.05.012},
url = {https://doi.org/10.1016%2Fj.cels.2018.05.012},
year = 2018,
month = {jun},
publisher = {Elsevier {BV}},
volume = {6},
number = {6},
pages = {752--758.e1},
author = {B{\'{e}}r{\'{e}}nice Batut and Saskia Hiltemann and Andrea Bagnacani and Dannon Baker and Vivek Bhardwaj and Clemens Blank and Anthony Bretaudeau and Loraine Brillet-Gu{\'{e}}guen and Martin {\v{C}}ech and John Chilton and Dave Clements and Olivia Doppelt-Azeroual and Anika Erxleben and Mallory Ann Freeberg and Simon Gladman and Youri Hoogstrate and Hans-Rudolf Hotz and Torsten Houwaart and Pratik Jagtap and Delphine Larivi{\`{e}}re and Gildas Le Corguill{\'{e}} and Thomas Manke and Fabien Mareuil and Fidel Ram{\'{\i}}rez and Devon Ryan and Florian Christoph Sigloch and Nicola Soranzo and Joachim Wolff and Pavankumar Videm and Markus Wolfien and Aisanjiang Wubuli and Dilmurat Yusuf and James Taylor and Rolf Backofen and Anton Nekrutenko and Björn Grüning},
title = {Community-Driven Data Analysis Training for Biology},
journal = {Cell Systems}
}
Congratulations on successfully completing this tutorial!
Developing GTN training material
This tutorial is part of a series to develop GTN training material, feel free to also look at: