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Visualisations in Galaxy

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Presenter notes contain extra information which might be useful if you intend to use these slides for teaching.

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Requirements

Before diving into this slide deck, we recommend you to have a look at:

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What is Data Visualisation

  • Making graphics to clearly convey data
  • Many different options

Selection of numerous graph types include pie, dot, bar, line charts, map and guages.

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What is Data Visualisation

  • More than just scatter plots and bar charts
  • Many domain-specific
    • Protein folding, network graphs, trees, genome browsers

Examples of 6 different visualisations in galaxy features an MSA, a couple graphs, RNA structure, and 3d visualisations of proteins

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Why visualizations?

  • "A picture is worth 1000 words"

  • Anscombe's Quartet

  • All these datasets share the same basic statistics:

    X mean: 54.26, X SD: 16.76
    Y mean: 47.83, Y SD: 26.93
    Correlation: -0.06
  • But visualisation reveals vastly different underlying structures!

Animated gif cycling through Anscombe's quartet and various variations that all have the same mean, SD, and correlation but look vastly different to someone's eyes.

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Anscombes quartet, all these datasets have same mean, standard deviation and correlation, yet when visualizing them it is revealed they have vastly different structures.

Data Visualisation in Galaxy

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Genome Browsers

  • Visualize the genome
    • mapped sequence reads, variants, genes, ..

JBrowse screenshot

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Genome Browsers in Galaxy

  • As display applications

    • e.g. UCSC browser, IBV, IGB, ..
    • accessed via links on dataset

    screenshot of display options

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JBrowse

  • Genome browser as a regular tool
  • Configure your settings

Screenshot of Galaxy showing the configuration interface for JBrowse.

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  • JBrowse is not a display application like the previous examples
  • Regular Galaxy tool

JBrowse

  • Then interactively explore your genome

Screenshot of JBrowse showing sequence, some bar charts, a SNP map highlighting snps, and aligned reads.

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Galaxy Charts

  • Accessed through Charts icon galaxy-barchart on dataset
  • Different options depending on datatype

Example of list of Galaxy charts

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Galaxy Charts

  • After selecting chart type, can customize settings
  • Example: Protein Viewer

    Configuration for the protein viewer is shown on the left, a 3d structure of a protein shown on the right

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Visualisation Tools

  • Many tools for visualisation
  • Example: Circos for circular data plots

Sample Circos images

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Thank You!

This material is the result of a collaborative work. Thanks to the Galaxy Training Network and all the contributors!

Galaxy Training Network

This material is licensed under the Creative Commons Attribution 4.0 International License.

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Requirements

Before diving into this slide deck, we recommend you to have a look at:

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